Curated Model: Rho GTPase regulation by RhoGDI/GAP interactions

Publication

Ota, T., Maeda, M., Okamoto, M., & Tatsuka, M. (2015).
Positive regulation of Rho GTPase activity by RhoGDIs as a result of their direct interaction with GAPs.
BMC systems biology, 9 (1), 3.

PMID: 25628036  DOI: 10.1186/s12918-015-0143-5

Description

This model describes how GEFs and GAPs interact with GDIs in Rho GTPase signaling. GDIs have a negative role in Rho GTPase signaling, as they inhibit GEFs, thereby suppressing overall Rho activity. GDIs also have a positive role, in which they inhibit GAPS under specific conditions, allowing sustained Rho activation.

The curated model

VCell reaction diagram

The model with varying GDI activity

SBGN visualizations
(from publication)
Simulations
(from publication)
Simulations
(from VCell)
Descriptions
In this canonical application, GDIs inhibit GEF and GAP activities by sequestering GTPase. Kinetic law was adjusted to eliminate effect of GDI on both GEF and GAP.

Figure 1A
In this GDI-integrated application, GDIs inhibit GEF and GAP activities by sequestering GTPase and by interacting with GEFs and GAPs.

Figure 1B
In this GDI-integrated application, GDI/GEF interaction was removed. Kinetic law was adjusted to eliminate effect of GDI on GEF.

Figure 1C
In this GDI-integrated application, GDI/GAP interaction was removed. Kinetic law was adjusted to eliminate effect of GDI on GAP.

Figure 1D

Downloads

VCell curated model CM_PM25628036.vcml designed with VCell
SBML original CM_PM25628036.xml Publication
GraphML CM_PM25628036.graphml exported from VANTED (SBGN-ED)
SBGN-ML CM_PM25628036.sbgn exported from CellDesigner

ModelBricks

  1. GEF activation
  2. GDI effects
  3. RhoGTPase cycle
  4. GDI binding

Contributors

John Albanese, Ann Cowan, Michael Blinov